Prepacked ROTI^®Dex-FPLC columns for size exclusion chromatography using syringe, pump or FPLC systems, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.

The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.

In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.

The column bed consists of ROTI^®Dex-25 Superfine. It is the gel filtration medium with the smallest dextran particles, which provide efficient results and very high resolutions. Biomolecules >5 kDa or >10 bp and particles >2 nm are purified. The fractionation range for proteins/peptides/biomolecules is between 1 and 5 kDa, with the highest resolution (most efficient separation) achieved between biomolecules >5 kDa and <1 kDa.

Sample volumes of 0.05-0.3 ml are recommended for the 1 ml columns.

Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.