Recombinant Mouse LAG3 Protein (His Tag)(Active)

Sizes: 10μg, 50μg

Catalogue Numbers: PKSM041104-10, PKSM041104-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: LAG3

Target Symptom: Lymphocyte activation gene 3 protein; CD223; Lag3

Target Species: Mouse

Expression Host: HEK293 Cells

Application: ELISA

Fusion Tag: C-6His

UNIProt ID: Q61790

Accession: Q61790

Background: Lymphocyte-activation gene 3 (LAG3), also known as CD223, is a type I transmembrane protein with four extracellular Ig-like domains, designated D1 to D4 and belongs to the immunoglobulin superfamily. The gene for LAG3 lies adjacent to the gene for CD4 on human chromosome 12p13.32 and shares approximately 20% identical to the CD4 gene. LAG3 is expressed on activated T cells, natural killer cells, B cells and plasmacytoid dendritic cells. LAG3 binds with high affinity to MHC class II molecules, and it interferes competitively with the binding of CD4 to MHC class II and thereby blocks the transduction of stimulatory signals mediated by this interaction. LAG3 negatively regulates cellular proliferation, activation, and homeostasis of T cells, and plays an important role in Treg suppressive function. LAG3 is the target of various drug development programs to develop new treatments for cancer and autoimmune disorders. The soluble form, sLAG-3, is being developed as a cancer drug.

Activity: Immobilized Mouse LAG-3-His at 10μg/ml(100 μl/well) can bind Biotinylated Human FGL1-Avi. The ED50 of Mouse LAG-3-His is 9.4 ug/ml.

Sequence: Ser23-Leu442

Purity: > 95 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 46.2 kDa

Observed MW: 55-80 kDa

Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Research Use Only